Effects of Nd:YAG laser irradiation on morphometry and lung function in elastase-induced emphysema in rats

Lasers Surg Med. 1998;23(4):204-12. doi: 10.1002/(sici)1096-9101(1998)23:4<204::aid-lsm3>3.0.co;2-9.

Abstract

Background & objective: Although thoracoscopic laser ablation therapy has been hailed as an effective surgical treatment for diffuse emphysema, no one has as yet made an in-depth study of the efficacy of this treatment. This investigation was undertaken to research the effects of laser pneumoplasty on an animal model of emphysema.

Study design/materials and methods: Eight weeks after elastase treatment, the rats' left lungs were irradiated using contact Nd:YAG laser. Pulmonary function tests were performed 4 weeks after irradiation and the lungs were prepared for histologic examination.

Results: Dense fibrous scars beneath the pleura were observed at 4 weeks after irradiation. Although mean linear intercept values of irradiated lungs were not much lower than those in the non-irradiated elastase-treated group, laser irradiation caused a significant decrease in lung volume. While there was no significant difference in quasistatic compliance, elastic recoil pressure of the lung increased to control levels at total lung capacity volume.

Conclusion: We conclude that laser therapy does not cause normalization of compliance, or improvement in the deeper part of the emphysematous lung, but rather a peripheral volume reduction and "encasement effect" on the lungs as a result of fibrotic scars.

Publication types

  • Comparative Study

MeSH terms

  • Analysis of Variance
  • Animals
  • Disease Models, Animal
  • Laser Therapy* / methods
  • Laser Therapy* / statistics & numerical data
  • Lung / pathology
  • Lung / physiopathology
  • Lung / surgery*
  • Male
  • Pancreatic Elastase
  • Pulmonary Emphysema / chemically induced
  • Pulmonary Emphysema / pathology
  • Pulmonary Emphysema / physiopathology
  • Pulmonary Emphysema / surgery*
  • Rats
  • Rats, Wistar
  • Respiratory Function Tests / statistics & numerical data
  • Thoracotomy

Substances

  • Pancreatic Elastase