Spi-1/PU.1 proto-oncogene induces opposite effects on monocytic and erythroid differentiation of K562 cells

Biochem Biophys Res Commun. 1998 Nov 18;252(2):383-91. doi: 10.1006/bbrc.1998.9587.

Abstract

Spi-1/PU.1 is a hematopoietic transcription factor of the Ets family. To analyze the effects of ectopic expression of spi-1 on the proliferation/differentiation of human myeloid leukemia cells, K562 cells were stably transfected with a spi-1 expression vector. The transfected cell lines expressed elevated levels of spi-1 mRNA and protein and high Spi-1-DNA binding activity. The spi-1 transfected cells showed reduced growth rates and reduced clonogenic cell growth. When the erythroid and monocytic differentiation markers were analyzed, spi-1 overexpression resulted in opposite effects: erythroid differentiation was significantly inhibited in spi-1 transfectants, while spi-1 overexpression increased the monocytic differentiation of cells. These results indicate a differential role of Spi-1 on the differentiation of human myeloid leukemia cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / metabolism
  • Cell Differentiation / drug effects
  • Cell Division
  • Erythropoiesis / drug effects
  • Erythropoiesis / genetics*
  • Genetic Vectors
  • Hematopoiesis / drug effects
  • Hematopoiesis / genetics*
  • Hematopoiesis / immunology
  • Humans
  • K562 Cells
  • Monocytes / cytology*
  • Monocytes / drug effects
  • Monocytes / metabolism*
  • Proto-Oncogene Mas
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins / metabolism
  • Proto-Oncogenes*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Tetradecanoylphorbol Acetate / pharmacology
  • Trans-Activators / genetics*
  • Trans-Activators / metabolism
  • Transfection
  • Vimentin / biosynthesis

Substances

  • Antigens, CD
  • MAS1 protein, human
  • Proto-Oncogene Mas
  • Proto-Oncogene Proteins
  • RNA, Messenger
  • Trans-Activators
  • Vimentin
  • proto-oncogene protein Spi-1
  • Tetradecanoylphorbol Acetate