Long-distance PCR-based strategy for preparing knock-in vectors directly from ES cell genomic DNA

MeSH terms

• Amino Acid Substitution
• Animals
• Cell Line
• Cloning, Molecular
• DNA / genetics*
• DNA Primers
• Gene Targeting
• Genetic Vectors / genetics*
• Lipoprotein Lipase / genetics
• Mice
• Mice, Inbred Strains
• Mutagenesis, Site-Directed
• Polymerase Chain Reaction / methods*
• Recombinant Fusion Proteins / genetics
• Stem Cells / chemistry
• Stem Cells / cytology
• Stem Cells / metabolism*