Expeditious synthesis of a new hexasaccharide using transglycosylation reaction catalyzed by Bacillus (1-->3),(1-->4)-Beta-D-glucan 4-glucanohydrolase

Carbohydr Res. 1998 Sep;311(1-2):95-9. doi: 10.1016/s0008-6215(98)00212-2.

Abstract

Enzymatic hydrolysis of barley (1-->3),(1-->4)-beta-D-glucan using a recombinant (1-->3),(1-->4)-beta-glucanase from Bacillus licheniformis gives Glc beta 4Glc beta 3Glc isolated after acetylation in 49% yield. Conventional treatment produced the corresponding beta-fluoride which was carefully de-O-acetylated. A transglycosylation reaction with this substrate, catalyzed by the title enzyme, gave Glc beta 4Glc beta 3Glc beta 4Glc beta 4Glc beta 3Glc in 20% yield.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus / enzymology*
  • Carbohydrate Sequence
  • Catalysis
  • Cellulase / chemistry*
  • Glycosylation
  • Molecular Sequence Data
  • Oligosaccharides / chemical synthesis*

Substances

  • Oligosaccharides
  • Cellulase