Antibodies against hepatitis G (anti-HGV) and HGV RNA were detected for frozen stored sera collected from 102 plasma donors and those subjects with positive anti-HGV and HGV RNA were followed-up for three years to study prevalence of HGV infection in them and their end-results. Anti-HGV was detected by enzyme immunoassay (EIA) with embedded antigen of synthetic peptide derived from different function regions of HGV. HGV RNA was detected by reverse transcription polymerase chain reaction (RT-PCR) with primers derived from NS3 region of HGV. Results showed that 19.61 percent (20/102) and 17.65 percent (18/102) of the plasma donors were positive for HGV RNA and anti-HGV, respectively, and 24.51 percent of them were infected with HGV (either positive for anti-HGV or HGV RNA), while only 0.94 percent (1/106) positive in the controls. It suggests that plasma donation is an important risk factor for HGV infection. Negative conversion rates of HGV RNA and anti-HGV during three-year follow-up were only 35.00 and 11.11 percent, respectively (7/20 and 2/18), which indicated a tendency of chronic carriage status in HGV infection.