Structure refinement against synchrotron Laue data: strategies for data collection and reduction

Acta Crystallogr D Biol Crystallogr. 1998 May 1;54(Pt 3):367-77. doi: 10.1107/s0907444997011517.

Abstract

The synchrotron Laue technique has been applied to high-resolution structure refinement of the ribotoxin, restrictocin [Yang & Moffat (1996). Structure, 4, 837-852]. By employing carefully designed data-collection strategies and the data-reduction algorithms incorporated in the software system LaueView [Ren & Moffat (1995a). J. Appl. Cryst. 28, 461-481; Ren & Moffat (1995b). J. Appl. Cryst. 28, 482-493], a set of high-resolution Laue data with a completeness and accuracy comparable to excellent monochromatic data was obtained. Through detailed comparison with the monochromatic data and electron-density maps derived from the Laue data, optimum data-collection and reduction strategies were identified and the application of Laue diffraction techniques to conventional crystallographic refinement was demonstrated.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Algorithms*
  • Crystallography, X-Ray / methods*
  • Data Collection*
  • Data Interpretation, Statistical*
  • Models, Molecular
  • Molecular Sequence Data
  • Molecular Structure
  • Synchrotrons*

Associated data

  • PDB/1AGZ
  • PDB/R1AGZSF