Objective: Detecting the loss of heterozygosity in paraffin-embedded pancreatic cancer tissues.
Methods: Analysing loss of heterzygosity (LOH) of microsatellite DNA on chromosome loci 3,5,7,9 and 18 with PCR-SSLP-silver stain method in pancreatic cancer.
Results: In 45 sporadic pancreatic cancer samples and their paired control tissue, LOH was detected on site for D18S46( 18q21.1,31.0%), D18S474 (18q21.1, 20.0%), D9S176 (9q22-31,20.0%), D3S1234 (3p14.2,17.5%),D 3S1289 (3p21.1, 15.7%), D3S1481 (3p14.2,4.9%), D7S486 (7q22,3.6%), D5S365 (5q32, 3.2%) and D3S587 (3p24-26, 2.6%).
Conclusion: Different percentages of loss of heterzygosity on specific chromosomal regions were found, and the meaning of the results was discussed. Some key genes may play a role in the pathogenesis of pancreatic cancer.