Abstract
We have identified a cDNA encoding a 212 amino acid protein (Nm23-H5) with 27-31% identity to the human members of the nm23/nucleoside diphosphate (NDP) kinase gene family. The nm23-H5 gene is located on chromosome 5q23-31 and is transcribed as one main transcript of 1.1 kb which is highly and specifically expressed in testis, in the spermatogonia and early spermatocytes. Nm23-H5 possesses most of the residues conserved among NDP kinases plus an additional COOH-terminus end of 55 amino acids unique to this protein. However, under usual assay conditions, Nm23-H5 expressed in Escherichia coli did not exhibit NDP kinase activity. These results suggest that Nm23-H5 is specifically involved in early stages of spermatogenesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Chromosome Mapping
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Chromosomes, Human, Pair 5
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Cloning, Molecular
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DNA Primers
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DNA, Complementary
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Histones / genetics*
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Humans
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In Situ Hybridization
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Male
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Molecular Sequence Data
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Monomeric GTP-Binding Proteins*
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NM23 Nucleoside Diphosphate Kinases
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Nucleoside-Diphosphate Kinase / metabolism
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Polymerase Chain Reaction
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RNA, Messenger / genetics
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Sequence Homology, Amino Acid
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Spermatozoa / metabolism*
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Testis / cytology
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Testis / metabolism*
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Transcription Factors / genetics*
Substances
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DNA Primers
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DNA, Complementary
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Histones
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NM23 Nucleoside Diphosphate Kinases
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NME5 protein, human
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RNA, Messenger
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Transcription Factors
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NME1 protein, human
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Nucleoside-Diphosphate Kinase
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Monomeric GTP-Binding Proteins