Adult human cardiomyocytes coexpress vimentin and Ki67 in heart transplant rejection and in dilated cardiomyopathy

J Heart Lung Transplant. 1998 Aug;17(8):795-800.

Abstract

Background: The aim of this study was to investigate whether adult human cardiomyocytes may reexpress vimentin and whether this is linked to cellular activation.

Methods: Myocardial samples of 81 heart transplant recipients (n=183) and patients with dilated cardiomyopathy (n=10) were investigated by immunohistochemistry with the use of the marker molecule vimentin, the muscle-specific protein desmin, and Ki67, a marker for cell activation.

Results: Vimentin protein expression in cardiomyocytes was found in 28 samples of transplant recipients and 5 myocardial samples of patients with dilated cardiomyopathy. Coexpression of vimentin and Ki67 was found in 52 of 340 vimentin-positive cardiomyocytes.

Conclusions: We suggest that the vimentin/Ki67 coexpression indicates cell activation processes as the result of different growth stimuli.

MeSH terms

  • Adult
  • Cardiomyopathy, Dilated / metabolism*
  • Female
  • G1 Phase
  • Graft Rejection / metabolism*
  • Heart Transplantation*
  • Humans
  • Immunohistochemistry
  • Ki-67 Antigen / biosynthesis*
  • Male
  • Myocardium / metabolism*
  • Resting Phase, Cell Cycle
  • Vimentin / biosynthesis*

Substances

  • Ki-67 Antigen
  • Vimentin