We examined a group of people professionally at risk of exposure to low doses of ionizing radiation (altogether 49 individuals). Age, use of therapeutic drugs, work-related exposure to hazardous agents, previous exposures to diagnostic X-rays, such as patient and nuclear medical examination, were registered. For each individual, the occupational radiation burden received over the past period of 5 years was taken from the official personal records based on film dosimetry controlled every month. A matched group of controls was chosen among the administrative employees (40 individuals). The mean age of the studied population at the time of blood sampling was 49 years (range 24-69). The individuals were divided into groups according to risk of exposure and sex. The alkaline comet assay was used to measure DNA breaks and alkali-labile sites. We compared the mean tail moments, tail length and percentage of DNA in the tail. There was a significant difference between the control and hazard groups in DNA damage. Higher DNA damage was also found for men than for women in the control group. There was no relation of DNA damage to age either in control or hazard group. Additionally, analysis of distributions of tail moment values pointed to a considerable individual diversity even in the control group. Therefore, further investigations were necessary into the suitability of the comet assay as a biological dosimetry method; the results obtained so far warrant such investigations.