Immune response to baculovirus expressed protein fragment amino acids 190-289 of respiratory syncytial virus (RSV) fusion protein

Vaccine. 1998 Jul;16(11-12):1127-30. doi: 10.1016/s0264-410x(98)80109-6.

Abstract

At least two neutralizing epitopes have been identified in the amino acid (aa) sequence 190-289 of the RSV fusion protein. The authors expressed this region in insect cells (bF190-289) and compared the immune response to bF190-289 with that induced by baculovirus expressed full-length fusion protein (bF). As with bF, mice primed with bF190-289 produced exclusively antibodies of IgG1 isotype, generated neutralizing antibodies, reduced significantly the virus titer (about a half log10 reduction) after RSV challenge and induced a Helper T (Th) 2 cell response in mediastinal lymph node cells (MLNC) restimulated in vitro. Thus, the aa sequence 190-289 represents a major immunogenic region of the RSV fusion protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antibodies, Viral / biosynthesis*
  • Baculoviridae*
  • Female
  • Immunoglobulin G / blood
  • Mice
  • Mice, Inbred BALB C
  • Peptide Fragments / immunology*
  • Respiratory Syncytial Viruses*
  • Viral Fusion Proteins / chemistry
  • Viral Fusion Proteins / immunology*

Substances

  • Antibodies, Viral
  • Immunoglobulin G
  • Peptide Fragments
  • Viral Fusion Proteins