In the central human retina, there are estimated to be nearly two L cone photoreceptors for each M cone. The extent to which this value varies across individuals is unclear and little is known about how the M:L cone ratio might change with retinal location. To address these questions, the ratio of M:L cone pigment mRNA was examined at different locations. For patches of central retina, the average M:L ratio was about 2:3 which decreased to about 1:3 for patches 40 degrees eccentric. There were also large individual differences among the 23 eyes examined. The extremes differed in central M:L mRNA ratio by a factor of > 3. The measured differences in mRNA ratio are proposed to reflect differences in photoreceptor ratio. Such variations provide unique opportunities for understanding how the neural circuitry for color vision is affected by changes in cone ratio.