Cloning, characterization, and expression of the CIP2 gene induced under cadmium stress in Candida sp

FEMS Microbiol Lett. 1998 May 15;162(2):325-30. doi: 10.1111/j.1574-6968.1998.tb13016.x.

Abstract

We isolated, sequenced, and expressed a cadmium-induced gene, CIP2, that specifically hybridizes to a mRNA of approximately 950 nucleotides. The CIP2 mRNA was barely present in normal Candida cells, but accumulated at higher levels in cadmium-treated cells. Other heavy metals such as copper, mercury, lead, and manganese had no effect on the expression of the CIP2 gene. CIP2 contains an open reading frame encoding a protein of 203 amino acids. This gene was also activated by an oxidant, diamide. Our results suggest that CIP2 may play a crucial role in the specific cellular response to oxidative stress evolved by the cadmium treatment.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Cadmium / toxicity*
  • Candida / drug effects*
  • Candida / genetics
  • Genes, Fungal / drug effects*
  • Genes, Fungal / genetics
  • Molecular Sequence Data

Substances

  • Cadmium

Associated data

  • GENBANK/AJ001180