Preparation of heat-labile antigens on air-dried cytologic specimens for immunocytochemical analysis

Acta Cytol. 1998 May-Jun;42(3):707-15. doi: 10.1159/000331831.

Abstract

Objective: To demonstrate the efficacy of Zamboni's solution and storage medium for immunocytochemistry of Ki-67, p53 tumor suppressor gene product and CD3 on long-preserved, air-dried cytologic specimens.

Study design: Air-dried imprint cytologic specimens obtained from 13 lymphatic tissues and 13 colorectal cancers were fixed with Zamboni's solution and stored for various periods in storage medium including 50% glycerol at -10 to -20 degree C. Mouse monoclonal antibody PC, PAb 1801 and anti-Leu-4 were used for immunostaining.

Results: Immunocytochemical identification of Ki-67, p53 and CD3 was possible on specimens stored for 36, 32 and 16 weeks, respectively.

Conclusion: Our method, using Zamboni's solution and storage medium, is feasible for immunocytochemistry on long-preserved, air-dried cytologic specimens using the three antibodies were employed.

MeSH terms

  • Air
  • Animals
  • Antibodies, Monoclonal / immunology
  • Antigens, Neoplasm / analysis*
  • Antigens, Neoplasm / immunology
  • Biomarkers, Tumor / analysis*
  • Biomarkers, Tumor / immunology
  • CD3 Complex / analysis*
  • CD3 Complex / immunology
  • Colorectal Neoplasms / chemistry*
  • Colorectal Neoplasms / pathology
  • Desiccation
  • Fixatives
  • Hot Temperature
  • Humans
  • Immunoenzyme Techniques*
  • Ki-67 Antigen / analysis*
  • Ki-67 Antigen / immunology
  • Lymph Nodes / chemistry
  • Lymph Nodes / pathology
  • Lymphoid Tissue / chemistry*
  • Lymphoid Tissue / pathology
  • Mice
  • Palatine Tonsil / chemistry
  • Palatine Tonsil / pathology
  • Preservation, Biological / methods*
  • Protein Denaturation
  • Specimen Handling / methods*
  • Time Factors
  • Tumor Suppressor Protein p53 / analysis*
  • Tumor Suppressor Protein p53 / immunology

Substances

  • Antibodies, Monoclonal
  • Antigens, Neoplasm
  • Biomarkers, Tumor
  • CD3 Complex
  • Fixatives
  • Ki-67 Antigen
  • Tumor Suppressor Protein p53