Abstract
Screening a Helicobacter pylori genomic library with antisera raised against H. pylori broth culture supernatant resulted in the identification of six antigens: urease, HspB, Lpp20, DnaK, MsrA, and a cysteine-rich 28-kDa protein (designated HcpA). H. pylori antigens may be released into the extracellular space by multiple mechanisms, including specific secretion pathways, autolysis, and membrane vesicle formation.
Publication types
-
Comparative Study
-
Research Support, U.S. Gov't, Non-P.H.S.
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Antigens, Bacterial / genetics
-
Antigens, Bacterial / metabolism*
-
Bacterial Proteins / genetics
-
Bacterial Proteins / metabolism
-
Escherichia coli Proteins*
-
Genomic Library
-
HSP70 Heat-Shock Proteins / genetics
-
HSP70 Heat-Shock Proteins / metabolism
-
Heat-Shock Proteins / genetics
-
Heat-Shock Proteins / metabolism
-
Helicobacter pylori / genetics
-
Helicobacter pylori / immunology*
-
Helicobacter pylori / metabolism
-
Hemolysin Proteins
-
Lipoproteins / genetics
-
Lipoproteins / metabolism
-
Membrane Transport Proteins*
-
Molecular Sequence Data
-
Plasmids / genetics
-
Sequence Analysis, DNA
-
Sequence Homology, Amino Acid
Substances
-
Antigens, Bacterial
-
Bacterial Proteins
-
Escherichia coli Proteins
-
HSP70 Heat-Shock Proteins
-
Hcp protein, Vibrio cholerae
-
Heat-Shock Proteins
-
Hemolysin Proteins
-
HspB protein, Helicobacter pylori
-
Lipoproteins
-
Lpp20 protein, Helicobacter pylori
-
Membrane Transport Proteins
-
msrA protein, Staphylococcus epidermidis
-
dnaK protein, E coli
Associated data
-
GENBANK/AF053708
-
GENBANK/AF053709
-
GENBANK/AF053710