Abstract
Three new human nuclear tRNA(Leu) genes have been isolated and sequenced using the PCR technique. Two of them represent genes containing a CAA anticodon and both contain introns of 22 nucleotides in length but differing in sequence. Intron-containing prolongated anticodon stems can be folded into a secondary structure similar to that of yeast pre-tRNA(Leu). The evolutionary conserved secondary structure suggests the same role of intron sequences in the human and yeast pre-tRNA(Leu) maturation pathway.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anticodon / genetics
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Base Sequence
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DNA Primers / genetics
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Evolution, Molecular
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Humans
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Introns
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Molecular Sequence Data
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Nucleic Acid Conformation
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Polymerase Chain Reaction
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RNA Precursors / chemistry
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RNA Precursors / genetics
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RNA Precursors / metabolism
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RNA Processing, Post-Transcriptional
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RNA, Fungal / chemistry
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RNA, Fungal / genetics
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RNA, Fungal / metabolism
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RNA, Transfer, Leu / chemistry
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RNA, Transfer, Leu / genetics*
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RNA, Transfer, Leu / metabolism
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Sequence Homology, Nucleic Acid
Substances
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Anticodon
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DNA Primers
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RNA Precursors
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RNA, Fungal
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RNA, Transfer, Leu