Polypyrimidine tract-binding protein interacts with HnRNP L

FEBS Lett. 1998 Apr 3;425(3):401-6. doi: 10.1016/s0014-5793(98)00269-5.

Abstract

Polypyrimidine tract-binding protein (PTB) is involved in pre-mRNA splicing and internal ribosomal entry site (IRES)-dependent translation. In order to identify cellular protein(s) interacting with PTB, we performed a yeast two-hybrid screening. Heterogeneous nuclear ribonucleoprotein L (hnRNP L) was identified as a PTB-binding protein. The interaction between PTB and hnRNP L was confirmed in an in vitro binding assay. Both PTB and hnRNP L were found to localize in the nucleoplasm, excepting the nucleoli, in HeLa cells by the green fluorescent protein (GFP)-fused protein detection method. The N-terminal half of PTB (aa 1-329) and most of hnRNP L (aa 141-558) is required for the interaction between PTB and hnRNP L.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA-Binding Proteins / metabolism*
  • Genes, Reporter / genetics
  • Green Fluorescent Proteins
  • HeLa Cells
  • Heterogeneous-Nuclear Ribonucleoprotein L
  • Heterogeneous-Nuclear Ribonucleoproteins
  • Humans
  • Luminescent Proteins / genetics
  • Microscopy, Fluorescence
  • Nuclear Proteins / metabolism
  • Polypyrimidine Tract-Binding Protein
  • Protein Biosynthesis / genetics
  • RNA Precursors / metabolism
  • RNA Splicing / physiology
  • RNA-Binding Proteins / metabolism*
  • Recombinant Fusion Proteins / metabolism
  • Ribonucleoproteins / metabolism*
  • Sequence Deletion / genetics
  • beta-Galactosidase / genetics
  • beta-Galactosidase / metabolism

Substances

  • DNA-Binding Proteins
  • Heterogeneous-Nuclear Ribonucleoprotein L
  • Heterogeneous-Nuclear Ribonucleoproteins
  • Luminescent Proteins
  • Nuclear Proteins
  • RNA Precursors
  • RNA-Binding Proteins
  • Recombinant Fusion Proteins
  • Ribonucleoproteins
  • Polypyrimidine Tract-Binding Protein
  • Green Fluorescent Proteins
  • beta-Galactosidase