In the development and administration of a certification examination in holistic nursing, an important issue is to ensure adequate content validity. Thus, the American Holistic Nurses' Association (AHNA) and the American Holistic Nurses' Certification Corporation (AHNCC), with the assistance of the National League for Nursing, conducted a practice analysis study to generate empirical data on the professional activities and knowledge required to practice holistic nursing on a day-to-day basis.