A light and electron microscopic study of cytoplasmic phospholipase A2 and cyclooxygenase-2 in the hippocampus after kainate lesions

Brain Res. 1998 Mar 30;788(1-2):223-31. doi: 10.1016/s0006-8993(97)01552-7.

Abstract

The systemic administration of kainate (10 mg/ml) into adult Wistar rats produces seizures and neurodegeneration. We have studied the effect of kainate administration on cPLA2 and COX-2 immunoreactivities after 3 days and 1, 2, 4 and 11 weeks. The cPLA2 immunoreactivity was increased in hippocampal neurons at 1 and 3 days after kainate injection suggesting that PLA2 may be involved in neurodegeneration. Increased cPLA2 and COX-2 immunoreactivities in astrocytes at 1, 2, 4 and 11 weeks after kainate injection indicate an adaptive astrocytic response that may be associated with gliosis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cyclooxygenase 2
  • Cytoplasm / enzymology*
  • Hippocampus / enzymology*
  • Immunohistochemistry
  • Injections, Intravenous
  • Isoenzymes / analysis*
  • Kainic Acid
  • Male
  • Microscopy / methods
  • Microscopy, Electron
  • Neurons / enzymology
  • Peroxidases / analysis*
  • Phospholipases A / analysis*
  • Phospholipases A2
  • Prostaglandin-Endoperoxide Synthases / analysis*
  • Rats
  • Rats, Wistar

Substances

  • Isoenzymes
  • Peroxidases
  • Cyclooxygenase 2
  • Prostaglandin-Endoperoxide Synthases
  • Phospholipases A
  • Phospholipases A2
  • Kainic Acid