TMC-49A, a novel transcriptional up-regulator of low density lipoprotein receptor, produced by Streptomyces sp. AS1345

J Antibiot (Tokyo). 1998 Feb;51(2):107-11. doi: 10.7164/antibiotics.51.107.

Abstract

Microbial metabolites were screened for a transcriptional up-regulator of low density lipoprotein (LDL) receptor by a reporter assay. TMC-49A was discovered as an up-regulator obtained from the fermentation broth of Streptomyces sp. AS1345. The structure of TMC-49A was elucidated to be butyl N-phenethylcarbamate by spectroscopic analyses. This compound enhanced the synthesis of LDL receptor in human hepatoma HepG2 cells as assessed by a receptor binding assay. Taxonomy of the producing strain is also described.

MeSH terms

  • Carbamates / chemistry
  • Carbamates / isolation & purification*
  • Carbamates / pharmacology*
  • Fermentation
  • Humans
  • Receptors, LDL / biosynthesis
  • Receptors, LDL / drug effects*
  • Receptors, LDL / metabolism
  • Streptomyces
  • Transcription, Genetic / drug effects*
  • Tumor Cells, Cultured / drug effects*
  • Tumor Cells, Cultured / metabolism
  • Up-Regulation / drug effects
  • Urethane / analogs & derivatives*

Substances

  • Carbamates
  • Receptors, LDL
  • TMC 49A
  • Urethane