MS-681a, b, c and d, new inhibitors of myosin light chain kinase from Myrothecium sp. KY6568. I. Characterization of producing strain and production, isolation and biological activities

J Antibiot (Tokyo). 1997 Dec;50(12):992-7. doi: 10.7164/antibiotics.50.992.

Abstract

Novel compounds MS-681a, b, c and d were isolated from the culture broth of a fungal strain KY6568. The strain was identified as Myrothecium sp. from its morphological characteristics. MS-681a, b, c and d inhibited the activity of purified smooth muscle myosin light chain kinase with IC50 values of 0.11, 0.29, 0.095 and 0.26 microM, respectively. Cyclic AMP-dependent protein kinase, cyclic GMP-dependent protein kinase and protein kinase C were not inhibited at 100 microM by MS-681 compounds.

MeSH terms

  • Aminoisobutyric Acids / isolation & purification
  • Aminoisobutyric Acids / pharmacology
  • Enzyme Inhibitors / isolation & purification*
  • Enzyme Inhibitors / pharmacology*
  • Hypocreales / chemistry*
  • Hypocreales / classification
  • Myosin-Light-Chain Kinase / antagonists & inhibitors*
  • Myosin-Light-Chain Kinase / drug effects
  • Peptides / isolation & purification
  • Peptides / pharmacology
  • Spermidine / analogs & derivatives*
  • Spermidine / isolation & purification
  • Spermidine / pharmacology

Substances

  • Aminoisobutyric Acids
  • Enzyme Inhibitors
  • MS-681a
  • Peptides
  • Myosin-Light-Chain Kinase
  • Spermidine