Abstract
Human adrenomedullin (hADM), human calcitonin gene-related peptide (hCGRP), and salmon calcitonin (sCT)-activated adenylyl cyclase with EC50 values of 132, 764, and 0.5 nM, respectively, in human breast cancer cell line, T 47D. Treatment of T 47D cell membranes with near maximal concentrations of sCT, hADM and hCGRP had no additive effect on adenylyl cyclase activity. Salmon calcitonin (8-32)[sCT (8-32)], selective antagonist of calcitonin receptor, inhibited the activation of adenylyl cyclase by these three peptides. On the other hand, the putative ADM receptor antagonist, ADM (22-52), and CGRP receptor antagonist, CGRP (8-37), failed to inhibit ADM-, CGRP- or sCT-activated adenylyl cyclase. These results suggest that in T47D cells, both ADM and CGRP activated adenylyl cyclase through sCT receptors.
MeSH terms
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Adenylyl Cyclases / metabolism
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Adrenomedullin
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Breast Neoplasms / metabolism
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Calcitonin / pharmacology
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Calcitonin Gene-Related Peptide / pharmacology
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Calcitonin Gene-Related Peptide Receptor Antagonists
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Cell Membrane / drug effects
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Cell Membrane / metabolism
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Drug Interactions
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Enzyme Activation / drug effects
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Female
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Humans
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Membrane Proteins / antagonists & inhibitors
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Peptide Fragments / pharmacology
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Peptides / pharmacology*
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Receptors, Adrenomedullin
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Receptors, Calcitonin / antagonists & inhibitors
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Receptors, Calcitonin / drug effects*
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Receptors, Calcitonin / metabolism
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Receptors, Peptide*
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Tumor Cells, Cultured / drug effects
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Tumor Cells, Cultured / metabolism
Substances
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Calcitonin Gene-Related Peptide Receptor Antagonists
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Membrane Proteins
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Peptide Fragments
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Peptides
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Receptors, Adrenomedullin
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Receptors, Calcitonin
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Receptors, Peptide
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adrenomedullin (22-52)
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calcitonin gene-related peptide (8-37)
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Adrenomedullin
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salmon calcitonin
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Calcitonin
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Adenylyl Cyclases
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Calcitonin Gene-Related Peptide