Ring test for the determination of N-terminal valine adducts of styrene 7,8-oxide with haemoglobin by the modified Edman degradation technique

J Chromatogr B Biomed Sci Appl. 1997 Nov 21;702(1-2):77-83. doi: 10.1016/s0378-4347(97)00380-0.

Abstract

A ring-test was organised between three laboratories using different versions of the modified Edman degradation technique for the gas chromatographic-mass spectrometric determination of N-terminal valine adducts of styrene 7,8-oxide. The analyses were performed on a sample of human haemoglobin reacted in vitro with styrene 7,8-oxide and on a set of five haemoglobin samples from mice dosed by i.p. injection of styrene. Strong correlations between the haemoglobin adduct determinations of the different laboratories were observed. However, covariance analysis revealed different slopes for the dose-response curves, indicating differences for the calibration of the reference globin or reference peptide.

Publication types

  • Comparative Study
  • Multicenter Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkylation
  • Animals
  • Dose-Response Relationship, Drug
  • Epoxy Compounds / chemistry
  • Epoxy Compounds / metabolism*
  • Gas Chromatography-Mass Spectrometry / methods*
  • Hemoglobins / chemistry
  • Hemoglobins / metabolism*
  • Humans
  • Injections, Intraperitoneal
  • Male
  • Mice
  • Mice, Inbred Strains
  • Styrene
  • Styrenes / administration & dosage
  • Valine / chemistry
  • Valine / metabolism*

Substances

  • Epoxy Compounds
  • Hemoglobins
  • Styrenes
  • Styrene
  • styrene oxide
  • Valine