Amino acids and peptides. XXXII: A bifunctional poly(ethylene glycol) hybrid of fibronectin-related peptides

Biochem Biophys Res Commun. 1997 Dec 18;241(2):595-8. doi: 10.1006/bbrc.1997.7855.

Abstract

An amino acid type poly(ethylene glycol) (aaPPEG) was prepared and its application to a drug carrier was examined. The peptides, Arg-Gly-Asp (RGD) and Glu-Ile-Leu-Asp-Val (EILDV) which were reported as active fragments of Fibronectin (a cell adhesion protein), were conjugated with aaPEG (molecular weight, 10,000). The hybrid, RGD-aaPEG-EILDV, was prepared by a combination of the solid-phase method and the solution method. Antiadhesive activity of the peptides was not lost by its hybrid formation with the large aaPEG molecule. A mixture of RGD (0.43 mmol) and EILDV (0.43 mmol) did not demonstrate an antiadhesive effect, but the hybrid containing 0.43 mmol of each peptide did exhibit this effect.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carrier Proteins / pharmacology
  • Cell Adhesion / drug effects*
  • Cell Adhesion Molecules / pharmacology*
  • Drug Carriers / pharmacology
  • Fibronectins / pharmacology*
  • Oligopeptides / pharmacology*
  • Polyethylene Glycols / pharmacology*

Substances

  • Carrier Proteins
  • Cell Adhesion Molecules
  • Drug Carriers
  • Fibronectins
  • Oligopeptides
  • glutamyl-isoleucyl-leucyl-aspartyl-valine
  • Polyethylene Glycols
  • arginyl-glycyl-aspartic acid