G-protein-coupled receptors in Saccharomyces cerevisiae: high-throughput screening assays for drug discovery

Trends Biotechnol. 1997 Dec;15(12):487-94. doi: 10.1016/S0167-7799(97)01119-0.

Abstract

G-protein-coupled receptors are an important class of therapeutic drug targets by virtue of their roles in the regulation of diverse cellular functions. Recent advances in the expression of heterologous G-protein-coupled receptors in the yeast Saccharomyces cerevisiae have led to the development of sensitive and selective assays of their ligand-induced activation. Implementation of this new technology in the high-throughput screening of compound libraries has enabled the discovery of novel ligands for the G-protein-coupled somatostatin receptor. This article describes the broad applicability of the technology and its use in drug discovery.

Publication types

  • Review

MeSH terms

  • Drug Industry / trends*
  • GTP-Binding Proteins*
  • Receptors, Somatostatin*
  • Saccharomyces cerevisiae / chemistry*

Substances

  • Receptors, Somatostatin
  • GTP-Binding Proteins