Abstract
The trans-sialidase from the trypomastigote stage of Trypanosoma cruzi was metabolically labeled with [3H]-palmitic acid and purified by immunoprecipitation with a monoclonal antibody. The action of PI-PLC on the immunoprecipitate released a lipid that was analyzed by TLC. Lyso-1-O-hexadecylglycerol and N-palmitoyl-sphinganine were obtained in a 1:3 ratio. A comparison with the GPI anchors present in the different stages of T. cruzi was made.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Chromatography, Thin Layer
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Glycosylphosphatidylinositols / metabolism*
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Membrane Lipids / metabolism*
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Mice
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Neuraminidase / metabolism*
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Phosphatidylinositol Diacylglycerol-Lyase
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Phosphoinositide Phospholipase C
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Substrate Specificity
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Trypanosoma cruzi / enzymology*
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Type C Phospholipases / metabolism
Substances
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Glycosylphosphatidylinositols
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Membrane Lipids
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Type C Phospholipases
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Phosphoinositide Phospholipase C
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Neuraminidase
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Phosphatidylinositol Diacylglycerol-Lyase