Isolation and characterization of an Azotobacter vinelandii algK mutant

FEMS Microbiol Lett. 1997 Nov 1;156(1):101-6. doi: 10.1111/j.1574-6968.1997.tb12712.x.

Abstract

Random Tn5 mutagenesis over Azotobacter vinelandii mucoid strain ATCC 9046 produced strain LA21, a non-mucoid, non-encysting mutant, carrying the Tn5 insertion within a gene homologous to algK from Pseudomonas aeruginosa encoding a periplasmic protein. algK, algJ and algG were shown to be transcribed as part of the palg8-alg44-algK-algJ-algG operon. A non-polar algK mutant was constructed and showed a non-mucoid phenotype, indicating that algK is essential for alginate production.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alginates / metabolism
  • Azotobacter vinelandii / genetics*
  • Azotobacter vinelandii / metabolism
  • Azotobacter vinelandii / ultrastructure
  • Bacterial Proteins / genetics*
  • Base Sequence
  • Chromosome Mapping
  • Desiccation
  • Genes, Bacterial*
  • Glucuronic Acid
  • Hexuronic Acids
  • Microscopy, Electron
  • Multigene Family
  • Mutagenesis, Insertional
  • Mutation*
  • Operon
  • Phenotype
  • Plasmids / genetics
  • Promoter Regions, Genetic
  • Pseudomonas aeruginosa / genetics
  • Transcription, Genetic

Substances

  • AlgK protein, Pseudomonas aeruginosa
  • Alginates
  • Bacterial Proteins
  • Hexuronic Acids
  • Glucuronic Acid