Abstract
We determined partial sequences of the gyrA and parC genes of the fluoroquinolone-susceptible strain ATCC 27853 and 22 clinical isolates of Pseudomonas aeruginosa. While a single amino acid change in GyrA with or without a change in ParC was found in 14 isolates with decreased susceptibility to fluoroquinolones, 3 higher-level fluoroquinolone-resistant isolates had a double amino acid change in GyrA and a single amino acid change in ParC.
MeSH terms
-
Amino Acid Sequence
-
Anti-Infective Agents / pharmacology*
-
Base Sequence
-
DNA, Bacterial / drug effects
-
DNA, Bacterial / genetics
-
Drug Resistance, Microbial / genetics
-
Fluoroquinolones
-
Genes, Bacterial / genetics*
-
Molecular Sequence Data
-
Mutation / physiology*
-
Polymerase Chain Reaction
-
Pseudomonas aeruginosa / drug effects*
-
Pseudomonas aeruginosa / genetics*
Substances
-
Anti-Infective Agents
-
DNA, Bacterial
-
Fluoroquinolones