Primer/template-independent synthesis of poly d(A-T) by Taq polymerase

K Hanaki; T Odawara; T Muramatsu; Y Kuchino; M Masuda; K Yamamoto; C Nozaki; K Mizuno; H Yoshikura

doi:10.1006/bbrc.1997.7197

Primer/template-independent synthesis of poly d(A-T) by Taq polymerase

Biochem Biophys Res Commun. 1997 Sep 8;238(1):113-8. doi: 10.1006/bbrc.1997.7197.

Authors

K Hanaki¹, T Odawara, T Muramatsu, Y Kuchino, M Masuda, K Yamamoto, C Nozaki, K Mizuno, H Yoshikura

Affiliation

¹ Department of Bacteriology, Faculty of Medicine, University of Tokyo, Japan.

PMID: 9299462
DOI: 10.1006/bbrc.1997.7197

Abstract

Taq DNA polymerase polymerized dATP and dTTP to poly d(A-T) without requiring added primer/template in the temperature range of 60-70 degrees C. Tth DNA polymerase also catalyzed the reaction, while delta Tth, Vent, Vent(exo-), Pfu, Ultma, BcaBEST, and KOD DNA polymerases did not. The reaction was distinct from the template-nonrequiring terminal deoxynucleotidyl transferase reaction which absolutely required primers.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA Nucleotidylexotransferase / metabolism
DNA Primers / metabolism*
DNA-Directed DNA Polymerase / metabolism*
Deoxyadenine Nucleotides / metabolism
Kinetics
Poly dA-dT / biosynthesis*
Poly dA-dT / chemistry
Substrate Specificity
Taq Polymerase
Templates, Genetic
Thymine Nucleotides / metabolism

Substances

DNA Primers
Deoxyadenine Nucleotides
Thymine Nucleotides
Poly dA-dT
Taq Polymerase
DNA Nucleotidylexotransferase
DNA-Directed DNA Polymerase
thymidine 5'-triphosphate