The purpose of our experiment was to study plasma immunoreactive galanin in the rat after swimming. Four groups of rats were used. At rest, one group was studied after an intravenous injection of D-glucose while another group received a corresponding saline injection. The two remaining groups, treated respectively with glucose and saline, were investigated after a 30-min swimming session. After 30 min of rest in their respective cages, or after swimming, the animals were anesthetized for immediate blood sampling. The main observation was that plasma galanin was higher after swimming than after rest only in glucose-treated rats, in the vena cava (11.82 +/- 2.90 vs. 5.05 +/- 1.65 pM) and the portal vein (15.75 +/- 3.74 vs. 6.58 +/- 1.75 pM). Both saline- and glucose-treated groups had a significant increase of plasma norepinephrine from rest to swimming in the vena cava. A decrease of plasma insulin was observed in the portal vein of exercised glucose-treated rats, while plasma glucose was higher in the portal vein of this group. In conclusion, after swimming, plasma galanin level was increased only in glucose-treated rats. A significant correlation was also observed between plasma galanin and glucose (r = 0.69, p < 0.01), suggesting that the presence of glucose is necessary to induce galanin liberation during exercise.