Ten 3-benzyl-5-substitued tetrahydro-2H-1,3,5-thiadiazine-2-thiones were synthesized and seven of them are reported for the first time. The structures of the compounds have been elucidated by UV, IR, H-NMR and elemental analysis. The in vitro activity of the compounds against 6 kinds of bacteria and 2 kinds of fungi was tested. The antimicrobial activities of all compounds are more potent than sulfadiazine sodium and less potent than norfloxacini. All compounds were found to be more active against gram-negative and less active against gram-positive bacteria and weak against fungi.