Enhancing effect of interleukin-4 on the secretion of interferon-gamma by alpha s1-casein-specific CD8+ T cells

Biosci Biotechnol Biochem. 1997 Jul;61(7):1156-62. doi: 10.1271/bbb.61.1156.

Abstract

alpha s1-Casein-specific CD8+ T cell clones expressed the interleukin (IL)-4 receptor, although they did not secrete detectable IL-4. We found that IL-4 significantly enhanced the secretion of interferon (IFN)-gamma by these CD8+ T cell clones. IL-4 also enhanced the secretion of IFN-gamma induced by stimulating the immobilized anti-CD3 antibodies of polyclonal CD8+ T cells which had been isolated from lymph nodes and were stimulated in vitro with the immobilized anti-CD3 antibody and IL-2. In addition, IL-4 added at the time of this first in vitro stimulation induced strong IFN-gamma productivity, as well as IL-4 and IL-10 productivity, which were detectable upon restimulation of these cells. Results are discussed in relation to the inhibitory effects of IFN-gamma production on IL-4-producing cells.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Antigens, CD / drug effects
  • Antigens, CD / metabolism
  • CD3 Complex / pharmacology
  • CD8-Positive T-Lymphocytes / drug effects*
  • CD8-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / metabolism*
  • Caseins / metabolism*
  • Caseins / pharmacology
  • Clone Cells
  • Female
  • Interferon-gamma / drug effects
  • Interferon-gamma / metabolism*
  • Interleukin-2 / pharmacology
  • Interleukin-4 / pharmacology*
  • Lymph Nodes / cytology
  • Mice
  • Mice, Inbred C57BL
  • Receptors, Interleukin / drug effects
  • Receptors, Interleukin / metabolism
  • Receptors, Interleukin-4

Substances

  • Antigens, CD
  • CD3 Complex
  • Caseins
  • Interleukin-2
  • Receptors, Interleukin
  • Receptors, Interleukin-4
  • Interleukin-4
  • Interferon-gamma