Planar chromatographic and liquid chromatographic analysis of thiamphenicol in bovine and human plasma

J AOAC Int. 1997 Jul-Aug;80(4):746-50.

Abstract

A planar chromatographic (PC) method was developed to determine thiamphenicol in human and bovine plasma with florphenicol as internal standard. The performance of the method was compared with that of a liquid chromatographic method. Recovery of extraction method developed for plasma was 81.51 +/- 2.85%. Reproducibilities of the PC assay performed with various PC plates were also good.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Anti-Bacterial Agents / blood*
  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / isolation & purification
  • Calibration
  • Cattle
  • Chromatography, High Pressure Liquid
  • Chromatography, Liquid
  • Densitometry
  • Drug Residues / metabolism*
  • Humans
  • Reference Standards
  • Reproducibility of Results
  • Spectrophotometry, Ultraviolet
  • Thiamphenicol / analogs & derivatives
  • Thiamphenicol / blood*
  • Thiamphenicol / chemistry
  • Thiamphenicol / isolation & purification

Substances

  • Anti-Bacterial Agents
  • florfenicol
  • Thiamphenicol