Molecular epidemiology of Vibrio cholerae O1 isolates from Colombia

J Med Microbiol. 1997 Jul;46(7):611-6. doi: 10.1099/00222615-46-7-611.

Abstract

A total of 173 Vibrio cholerae O1 isolates from the recent cholera epidemic in Colombia was analysed by the polymerase chain reaction (PCR) for the genes encoding the A subunit of cholera toxin (ctxA) and the zonula occludens toxin (zot), and by ribotyping. All isolates were positive for ctx A and zot, which was confirmed by hybridisation. Ribotyping with restriction endonuclease Bg/I digestion of total DNA revealed three ribotypes: B5a comprising 165 (96.4%) isolates, and two new designated ribotypes B20 and B21a in six (3.5%) isolates and two (1.1%) isolates, respectively. These findings have significant public health implications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Cholera / epidemiology*
  • Cholera / microbiology
  • Cholera Toxin / genetics*
  • Colombia / epidemiology
  • Disease Outbreaks
  • Endotoxins
  • Genes, Bacterial
  • Humans
  • Molecular Epidemiology
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • RNA, Bacterial / genetics
  • RNA, Ribosomal / genetics*
  • Vibrio cholerae / classification
  • Vibrio cholerae / genetics*
  • Vibrio cholerae / isolation & purification
  • rRNA Operon

Substances

  • Endotoxins
  • RNA, Bacterial
  • RNA, Ribosomal
  • zonula occludens toxin, Vibrio cholerae
  • Cholera Toxin