Differences in phycoerythrin- or fluorescein-isothiocyanate conjugated 8G12 on CD34+ cell evaluation

Haematologica. 1997 May-Jun;82(3):334-5.

Abstract

To evaluate the equivalence of 8G12 conjugated with either phycoerythrin (PE) or fluorescein isothiocyanate (FITC), duplicate fluorochrome-8G12 labelling was carried out in 229 samples. Additionally, 53 samples were simultaneously immunostained with FITC-8G12 and PE-8G12. Significantly higher values (p < 0.001) were observed in PE-CD34+ cells when compared with FITC-CD34+ cells both in duplicate and simultaneous analysis. Our data suggest that the choice of fluorochrome is relevant in the measurement of CD34+ cells with 8G12.

Publication types

  • Comparative Study

MeSH terms

  • Antibodies, Monoclonal / chemistry*
  • Antibodies, Monoclonal / immunology
  • Antigens, CD34 / analysis*
  • Antigens, CD34 / immunology
  • Antineoplastic Agents / pharmacology
  • Cell Separation
  • Evaluation Studies as Topic
  • Flow Cytometry
  • Fluorescein-5-isothiocyanate*
  • Fluorescent Antibody Technique, Indirect / methods*
  • Fluorescent Dyes*
  • Granulocyte Colony-Stimulating Factor / pharmacology
  • Hematopoietic Stem Cells / chemistry*
  • Hematopoietic Stem Cells / drug effects
  • Humans
  • Leukapheresis
  • Leukocyte Count* / drug effects
  • Phycoerythrin*
  • Recombinant Proteins / pharmacology
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • Antibodies, Monoclonal
  • Antigens, CD34
  • Antineoplastic Agents
  • Fluorescent Dyes
  • Recombinant Proteins
  • Phycoerythrin
  • Granulocyte Colony-Stimulating Factor
  • Fluorescein-5-isothiocyanate