Theorectical knowledge and practical ability of nurses and nursing auxiliaries regarding methods of prevention and control of hospital acquired urinary tract acquired infection are studied in 29 hospitals of São Paulo. For this it was filled a form with questions constructed with questions for the correct form of prevention and control of urinary tract acquired infection, particularly those that were published for the CDC guidelines of the prevention and control of the Center of Disease Control (CDC). We also studied the possible effects of the infection control Department on the Theoretical Knowledge and practical ability of nurses and nursing auxiliaries regarding infection control measures.