Solid phase competitive luminescence immunoassay for immunoglobulin A in faeces: development and clinical validation

Clin Chim Acta. 1997 May 6;261(1):1-17. doi: 10.1016/s0009-8981(96)06510-2.

Abstract

We describe a new simple solid-phase competitive luminescence immunoassay (LIA) for the determination of immunoglobulin A (IgA) in faeces. The assay utilizes an anti-alpha-chain IgA antibody which is coated to polystyrene beads and acridinium ester-labelled human IgA as tracer and, therefore, measures both monomeric and polymeric IgA. Dilution recovery of an internal standard was 96, 100 and 103%. Interassay and intra-assay coefficients of variation (C.V.) ranged from 4.5 to 12.9%. The upper limit of normal of faecal IgA in 122 healthy controls was found to be 300 mg/l IgA (mean 73 mg/l, specificity of 99.2%). Patients with inactive Crohn's disease (Crohn's disease activity index (CDAI < 150, n = 14) had faecal IgA values up to 3317 mg/l (mean 1073 mg/l; P < 0.0001). In the active group (CDAI > 150, n = 26) faecal IgA values ranged from 49 to 4094 mg/l (mean 1253 mg/l; P < 0.0001). Patients with ulcerative colitis were divided into a group with active disease (n = 18) and a remission group (n = 16) with values up to 1843 mg/l faecal IgA (man 486 mg/l; P < 0.0032) and up to 602 mg/l faecal IgA (mean 176 mg/l; P < 0.4833), respectively. We also studied patients with non-inflammatory diseases of the gut with this assay. This LIA has proved to be a reliable method for the determination of elevated faecal IgA concentrations and for the detection of pathological findings in the gastrointestinal tract, especially in Crohn's disease.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Binding, Competitive
  • Colitis, Ulcerative / immunology
  • Colonic Polyps / immunology
  • Colorectal Neoplasms / chemistry
  • Colorectal Neoplasms / immunology
  • Crohn Disease / immunology
  • Diverticulum / immunology
  • Feces / chemistry*
  • Hemorrhoids / immunology
  • Humans
  • Immunoassay / methods*
  • Immunoassay / statistics & numerical data
  • Immunoglobulin A / chemistry*
  • Luminescent Measurements
  • Middle Aged
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • Immunoglobulin A