Rapid diagnosis of enterovirus infection by a new one-step reverse transcription-PCR assay

H H Kessler; B Santner; H Rabenau; A Berger; A Vince; C Lewinski; B Weber; K Pierer; D Stuenzner; E Marth; H W Doerr

doi:10.1128/jcm.35.4.976-977.1997

Rapid diagnosis of enterovirus infection by a new one-step reverse transcription-PCR assay

J Clin Microbiol. 1997 Apr;35(4):976-7. doi: 10.1128/jcm.35.4.976-977.1997.

Authors

H H Kessler¹, B Santner, H Rabenau, A Berger, A Vince, C Lewinski, B Weber, K Pierer, D Stuenzner, E Marth, H W Doerr

Affiliation

¹ Institute of Hygiene, KF-University Graz, Austria. harald.kessler@kfunigraz.ac.at

Abstract

The AMPLICOR Enterovirus Test was evaluated with 103 cerebrospinal fluid (CSF) specimens. Twenty-seven CSF specimens were culture positive. With the AMPLICOR test, enterovirus RNA was detected in 34 specimens. Compared with culture, the AMPLICOR test gave a sensitivity of 96.3% and a specificity of 100%. The sensitivity of culture was 79.4% in comparison with the AMPLICOR test.

MeSH terms

Enterovirus / isolation & purification*
Enterovirus Infections / diagnosis*
Humans
Polymerase Chain Reaction / methods*