The chemical composition of the essential oils obtained from the aerial parts of Helichrysum stoechas ssp. barrelieri and H. taenari was analysed by GC and GC/MS. From the thirty-nine identified constituents representing the 73.87% and 87.41% of the two oils, respectively, beta-elemene, beta-caryophyllene, geraniol, and camphene were the major components. Furthermore, it was found that the oils exhibited significant antibacterial activity against six Gram (+/-) bacteria.