Signal sequence trap. Expression cloning method for secreted proteins and type 1 membrane proteins

Methods Mol Biol. 1997:69:203-19. doi: 10.1385/0-89603-383-x:203.
No abstract available

MeSH terms

  • Animals
  • Antibodies
  • Base Sequence
  • COS Cells
  • Cloning, Molecular / methods*
  • DNA Primers
  • DNA, Complementary
  • Databases, Factual
  • Gene Library
  • Genes, Reporter
  • Humans
  • Indicators and Reagents
  • Membrane Proteins / biosynthesis*
  • Microscopy, Fluorescence / methods
  • Plasmids
  • Polymerase Chain Reaction / methods*
  • Protein Sorting Signals / biosynthesis*
  • Receptors, Interleukin-2 / analysis
  • Receptors, Interleukin-2 / biosynthesis*
  • Recombinant Fusion Proteins / analysis
  • Recombinant Fusion Proteins / biosynthesis*
  • Sequence Tagged Sites
  • Transfection / methods

Substances

  • Antibodies
  • DNA Primers
  • DNA, Complementary
  • Indicators and Reagents
  • Membrane Proteins
  • Protein Sorting Signals
  • Receptors, Interleukin-2
  • Recombinant Fusion Proteins