Flow cytometric investigation of non-specific erythrocyte antigens

Transfus Clin Biol. 1997;4(1):149-52. doi: 10.1016/s1246-7820(97)80033-x.

Abstract

Thirteen monoclonal antibodies submitted to the Third Workshop on Erythrocyte Antigens from the panel "non-specific erythrocyte antigens" were tested for their reactivity with different types of cells. Most of them were defined as specific for adhesion antigens. The CD 44 antibodies 2D3-1, 2D3-2, 2D3-3 and 2D3-4 reacted as expected for CD 44 except their negative reactivity with the myeloid cell line HL 60 and B-cell line Raji. The CD 47 antibodies 2D3-5 and 2D3-6 reacted specific. Only with Raji and T-cell line MOLT 4 the CD 58 antibodies 2D3-7 and 2D3-8 showed reactivity as expected which indicates that they are "CD 58 related". The CD 99 antibody 2D3-9 shows similar results as expected for a CD 99 specific antibody except its high reactivity against Raji. From the RBC-related antibodies 2D3-11 and 2D3-12 the latter becomes completely negative with trypsin treated erythrocytes. The antibody is negative on normal peripheral blood lymphocytes but reacts with transformed cell lines like Raji and MOLT 4. With a view to their reactivity to the cells tested at least 2D3-13 of the Rh-related antibodies seems to be similar to CD 47 antibodies.

MeSH terms

  • 12E7 Antigen
  • Agglutination Tests
  • Antibodies, Monoclonal
  • Antigens, CD / immunology
  • CD47 Antigen
  • CD58 Antigens / immunology
  • Carrier Proteins / immunology
  • Cell Adhesion Molecules / immunology
  • Cell Line
  • Epitopes*
  • Erythrocytes / immunology*
  • Flow Cytometry*
  • Humans
  • Hyaluronan Receptors / immunology
  • Isoantigens / immunology*
  • Rh-Hr Blood-Group System / immunology
  • Tumor Cells, Cultured

Substances

  • 12E7 Antigen
  • Antibodies, Monoclonal
  • Antigens, CD
  • CD47 Antigen
  • CD47 protein, human
  • CD58 Antigens
  • CD99 protein, human
  • Carrier Proteins
  • Cell Adhesion Molecules
  • Epitopes
  • Hyaluronan Receptors
  • Isoantigens
  • Rh-Hr Blood-Group System