High resolution imaging of native biological sample surfaces using scanning probe microscopy

Curr Opin Struct Biol. 1997 Apr;7(2):279-84. doi: 10.1016/s0959-440x(97)80037-1.

Abstract

The possibility of acquiring high resolution topographs using scanning probe microscopes under physiological conditions allows the observation of biomolecules at work. Progress has recently been made in imaging protein-DNA complexes, individual oligomers and protein arrays. Scanning probe microscopes are now tools that complement X-ray crystallography and electron microscopy.

Publication types

  • Review

MeSH terms

  • Bacterial Outer Membrane Proteins / ultrastructure
  • Crystallography, X-Ray
  • DNA / ultrastructure*
  • DNA-Binding Proteins / ultrastructure*
  • Macromolecular Substances
  • Microscopy / instrumentation
  • Microscopy / methods*
  • Microscopy, Electron
  • Plasmids
  • Protein Conformation
  • Surface Properties

Substances

  • Bacterial Outer Membrane Proteins
  • DNA-Binding Proteins
  • Macromolecular Substances
  • DNA