To test the applicability of microsatellite markers in the study of DNA amplifications evaluated by comparative genomic hybridization, we analyzed 55 highly polymorphic microsatellite marker loci from six liposarcoma tumors (seven specimens) and from one atypical lipoma with a gain or high-level amplification at 12q13-22. Twelve-trisomic neoplastic cells from a patient with B-cell chronic lymphocytic leukemia were used as a positive control, in which 74% of informative loci showed allelic imbalance. In every tumor specimen microsatellite marker loci analysis showed allelic imbalance. The amplicons were discontinuous, indicating the presence of separate amplicons in the 12q13-22 region. Not only gains but also losses as well as concomitant gains and losses of alleles were observed. The use of microsatellite markers has several advantages: gene loci as well as flanking DNA loci can be analyzed, it is fast and lends itself to automation, and allows a large number of marker loci to be analyzed simultaneously.