Abstract
CD45 is expressed on all B cells and has been reported to be essential for their B cell receptor mediated stimulation. The present study addressed T-cell-independent B cell responses in CD45-deficient mice using the glycoprotein (G) of vesicular stomatitis virus (VSV) as a model antigen. VSV-G exists in two forms exhibiting different degrees of repetitiveness. In mice, the two forms of VSV-G induce either a type 1 or a type 2 T-cell-independent B cell response. We found that CD45-deficient mice mounted T-cell-independent B cell responses to both forms of VSV-G. This demonstrates that the B cell receptor complex is able to generate a functional signal in the absence of CD45, provided the cross-linking stimulus is sufficiently strong.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Viral / biosynthesis
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Antigens, Viral
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B-Lymphocytes / enzymology
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B-Lymphocytes / immunology*
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Cross-Linking Reagents
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Gene Targeting
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Glycoproteins / immunology
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Immunoglobulin M / biosynthesis
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In Vitro Techniques
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Leukocyte Common Antigens / genetics
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Leukocyte Common Antigens / metabolism*
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Lymphocyte Activation
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Membrane Glycoproteins*
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Mice
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Mice, Mutant Strains
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Neutralization Tests
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Protein Tyrosine Phosphatase, Non-Receptor Type 1
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Receptors, Antigen, B-Cell / metabolism
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T-Lymphocytes, Helper-Inducer / immunology
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Vesicular stomatitis Indiana virus / immunology*
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Viral Envelope Proteins / immunology
Substances
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Antibodies, Viral
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Antigens, Viral
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Cross-Linking Reagents
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G protein, vesicular stomatitis virus
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Glycoproteins
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Immunoglobulin M
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Membrane Glycoproteins
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Receptors, Antigen, B-Cell
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Viral Envelope Proteins
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Leukocyte Common Antigens
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Protein Tyrosine Phosphatase, Non-Receptor Type 1