This study continues a series of investigations of the gene pool of native Siberian ethnic groups. In a population of Tundra Nentsi (Northern Samoyeds) and a group of Komi-Zyryans (Finno-Ugrian) (Samburg settlement, Tyumenskaya oblast, Yamalo-Nenetskii Autonomous okrug), gene markers of the following genetic systems were studied: blood groups (ABO, MNSs, Rhesus, Kell, Duffy, and P), erythrocyte acid phosphatase (AcP), phosphoglucomutase 1 (PGM 1), haptoglobin (Hp), and transferrin (Tf). The population of Samburg Tundra Nentsi was shown to have a close genetic relationship with the "core" of the Forest Nentsi population. In Northern Samoyeds, three carriers of the rare allele K (blood group Kell) were found for the first time. It is suggested that this allele was transferred into the population of Tundra Nentsi from Komi. Samburg Tundra Nentsi are found to have the maximum frequency of the allele PGM 1 (Posphoglucomutase 1) among aboriginal populations of northern Asia. Analysis of original data and the literature revealed a significant genetic distance between the Komi and Northern Samoyed populations. It was shown that Samburg Komi occupy an intermediate position between the clusters of Nenets populations and Finno-Ugrians (Komi) living in Komi Republic.