Changes in the diameters of individual follicular structures on ovaries were measured by transrectal ultrasonography for 29 to 40 days and the plasma concentrations of luteinising hormone (LH) follicle-stimulating hormone (FSH), progesterone and oestradiol-17 beta were determined in four cows with ovarian cysts. When these structures decreased in size, new follicular structures appeared and developed into cysts. Progesterone concentrations in plasma were below 1.0 ng ml-1 during the experimental periods. Plasma concentrations of oestradiol-17 beta fluctuated. The mean concentration of oestradiol-17 beta in plasma differed (P < 0.01) depending on the stage of the cyst. No preovulatory surges of LH were detected during the developmental stage of the cysts.