Abstract
A method based on 16S rRNA gene-targeted PCR and oligonucleotide probing was developed for detecting Mycobacterium chlorophenolicum PCP-1 in soil. The primers and probe were specific for PCP-1 in DNA extracts of three soils. The method allowed for PCP-1 detection in soil with a detection limit of 3 x 10(2) cells per g.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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DNA Primers / genetics
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DNA, Bacterial / genetics
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DNA, Ribosomal / genetics
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Evaluation Studies as Topic
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Gene Targeting
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Genes, Bacterial
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Molecular Sequence Data
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Mycobacterium / classification
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Mycobacterium / genetics*
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Mycobacterium / isolation & purification*
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Polymerase Chain Reaction / methods*
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Polymerase Chain Reaction / statistics & numerical data
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RNA, Bacterial / genetics*
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RNA, Ribosomal, 16S / genetics*
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Sensitivity and Specificity
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Sequence Homology, Nucleic Acid
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Soil Microbiology*
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Species Specificity
Substances
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DNA Primers
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DNA, Bacterial
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DNA, Ribosomal
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RNA, Bacterial
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RNA, Ribosomal, 16S