Biotin-labeled antigen: a novel approach for detection of Puumala virus-specific IgM

A Ivanov; O Vapalahti; H Lankinen; E Tkachenko; A Vaheri; B Niklasson; A Lundkvist

doi:10.1016/0166-0934(96)02090-3

Biotin-labeled antigen: a novel approach for detection of Puumala virus-specific IgM

J Virol Methods. 1996 Oct;62(1):87-92. doi: 10.1016/0166-0934(96)02090-3.

Authors

A Ivanov¹, O Vapalahti, H Lankinen, E Tkachenko, A Vaheri, B Niklasson, A Lundkvist

Affiliation

¹ Chumakov Institute of Poliomyelitis and Viral Encephalitides, Ac. Med. Sci., Moscow, Russia.

PMID: 8910652
DOI: 10.1016/0166-0934(96)02090-3

Abstract

A novel enzyme-linked immunosorbent assay, BLA IgM-ELISA, based on baculovirus-expressed Puumala (PUU) virus nucleocapsid protein, was developed for rapid diagnosis of nephropathia epidemica. The recombinant antigen (bac-PUU-N) was purified to homogeneity by HPLC and conjugated to biotin. The biotin-streptavidin system, in combination with the mu-capture technique, rendered the BLA IgM-ELISA a sensitivity similar to or higher than that of PUU virus IgM mu-capture ELISA based on native antigen. The assay was shown to be highly specific when evaluated using a panel of 160 patient and control sera.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Viral / analysis*
Antigens, Viral / immunology*
Biotin
Capsid / immunology*
Enzyme-Linked Immunosorbent Assay / methods*
Hantavirus Infections / diagnosis*
Hantavirus Infections / immunology
Immunoglobulin M / analysis
Orthohantavirus / immunology*
Recombinant Proteins / immunology

Substances

Antibodies, Viral
Antigens, Viral
Immunoglobulin M
Recombinant Proteins
Biotin