Distinction between genotypes of Lupinus species by sodium dodecyl sulphate-gel electrophoresis and by capillary electrophoresis

Electrophoresis. 1996 Jan;17(1):221-3. doi: 10.1002/elps.1150170137.

Abstract

Effective distinction was achieved among a wide range of lupin grain samples by either sodium dodecyl sulphate (SDS)-gel electrophoresis or capillary electrophoresis, based on grain-protein composition. Capillary electrophoresis was faster (< 1 h) and provided slightly greater distinction between the samples. On the other hand, SDS-gel electrophoresis could provide a greater through-put of samples in a 24 h period. Either technique could be used successfully to distinguish between lupin species and cultivars for taxonomic analysis or seed identification.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrophoresis, Capillary*
  • Electrophoresis, Polyacrylamide Gel*
  • Fabaceae / genetics*
  • Genotype
  • Microchemistry
  • Plant Proteins / analysis*
  • Plants, Medicinal*
  • Sensitivity and Specificity
  • Species Specificity

Substances

  • Plant Proteins