The recombinant 120-kilodalton protein of Ehrlichia chaffeensis, a potential diagnostic tool

J Clin Microbiol. 1996 Nov;34(11):2853-5. doi: 10.1128/jcm.34.11.2853-2855.1996.

Abstract

DNA encoding two repeat units of 120-kDa protein of Ehrlichia chaffeensis was cloned into the expression vector pGEX and expressed in Escherichia coli. The sensitivity and specificity of a dot blot assay for detection of human antibodies with the recombinant protein were 86 and 100%, respectively, compared with an indirect immunofluorescence assay.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Bacterial / blood
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / immunology
  • Bacteriological Techniques* / statistics & numerical data
  • Base Sequence
  • Cloning, Molecular
  • DNA Primers / genetics
  • DNA, Bacterial / genetics
  • Ehrlichia chaffeensis / genetics*
  • Ehrlichia chaffeensis / immunology
  • Ehrlichia chaffeensis / isolation & purification
  • Ehrlichiosis / diagnosis*
  • Ehrlichiosis / immunology
  • Ehrlichiosis / microbiology*
  • Escherichia coli / genetics
  • Evaluation Studies as Topic
  • Fluorescent Antibody Technique, Indirect / statistics & numerical data
  • Humans
  • Immunoblotting / methods*
  • Immunoblotting / statistics & numerical data
  • Molecular Weight
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Repetitive Sequences, Nucleic Acid
  • Sensitivity and Specificity

Substances

  • Antibodies, Bacterial
  • Bacterial Proteins
  • DNA Primers
  • DNA, Bacterial
  • Recombinant Proteins